Ramkumar
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Latest activity by Ramkumar-
Ramkumar created an article,
What is the shipping solvent for Lux column?
Lux amylose & cellulose columns are shipped in normal phase solvent Hexane: IPA (9:1). If these columns are to be analyzed in reverse phase or polar organic mode, a proper solvent switching should...
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Ramkumar created an article,
How does a polysaccharide stationary phase recognize chiral isomers?
Lux Polysaccharide stationary phases are cellulose or amylose coated silica with specific chiral selectors. The sugar itself brings a selectivity. For example, cellulose is a 1, 4-beta linkage of ...
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Ramkumar created an article,
What are some ways to control selectivity in a chiral separation?
There are two ways to effect a chiral separation. It can be effected by using a chiral discriminator or selector. The chiral selector can be added as an additive to the mobile phase or a column wi...
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Ramkumar created an article,
How to find the number of possible enantiomer for a given molecule?
The number of possible enantiomer is dependent on the chiral centers (asymmetric carbons). If there are n number of chiral centers, then 2n chiral isomers are possible. So, one chiral center would...
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Ramkumar created an article,
What are chiral isomers? How do I recognize Chiral centers?
A molecule and its non-superimposable mirror image is called enantiomer. A simple example to illustrate enantiomer is left and right hand are mirror images of each other and cannot be superimposed...
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Ramkumar created an article,
How is a ZB-guardian/ Z-guard column in GC different from a guard column in LC?
A ZB-guardian/ Z-guard column in GC acts a s a barrier that prevents nonvolatile analytes from entering into the analytical column. It also helps in peak focusing phenomena. So it acts both as a g...
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Ramkumar created an article,
How does ZB-guardian/ Z-guard act as retention gap column in a large volume injection?
In a large volume injection, 5-100µL of the solvent is injected into the GC inlet. The vapor volume of the solvent is approximately 1000 times the liquid volume (depending on the solvent the vapor ...
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Ramkumar created an article,
Do we need ZB-guardian/ Z-guard for split injection?
Split injection is a fast injection mode and the amount of solvent entering the column is less. In general, it does not need ZB-guardian/ Z-guard to act as a retention gap column. However, if there...
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Ramkumar created an article,
How does ZB-guardian/ Z-guard enhance the peak shape in a splitless injection?
In a splitless injection, the analytes and solvent enters the head of the ZB guardian/Z-guard as vapors. If the initial oven temperature is lower than the boiling point of the solvent, the analytes...
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Ramkumar created an article,
How does ZB-guardian/ Z-guard enhance the peak shape in an on column injection?
In an on column injection, the analytes and the sample are introduced as a liquid in the head of the column. Introducing a liquid directly to the head of the analytical column would show increased ...