Phenom Support Answers

A collection of questions that received good ratings and feedback from our customers.

• My SPE method doesn’t provide a good enough cleanup. What are some ways to improve the cleanup in my method?
• My spiked sample has good recoveries using my SPE method. Why do I get low recoveries when real samples are used?
• My sugar samples contain metal ions, will this cause a problem when using Rezex?
• Should I select 2.1 mm ID or 3.0 mm ID Kinetex columns for my LC-MS study?
• Should I use a jet separator for large ID capillary GC columns for GC/MS analysis?
• Should I use TFA or Formic Acid for my peptide separation on Aeris PEPTIDE?
• SPE Troubleshooting Series: I. Low recoveries
• SPE Troubleshooting Series: II. Extra Peak
• SPE Troubleshooting Series: III. Inconsistency
• SPE Troubleshooting Series: IV. Slow Flow
• The glass wool in my GC injection port liner shifts downwards after a few injections, causing problems with peak shape and quantitation. Is there anything I can do to solve this?
• The back pressure in my normal phase method is increasing, how can reduce it?
• The back pressure of my Axia column has increased, is it possible to change the inlet frit?
• The pressure has increased on my Yarra 1.8u X150, can I reverse the direction of flow through the column when cleaning it?
• The pressure reading on my HPLC is oscillating wildly with an isocratic mobile phase, what could be causing the issue?
• The retention times in my normal phase method are unstable throughout the day, what could be causing this?
• The roQ brochure says graphitized carbon black (GCB) is not for use with planar pesticides. Why and what are some examples of planar pesticides?
• The sensitivity of my electrochemical detector is decreasing, and the signal is becoming progressively more noisy, is there anything I can do to fix this?
• There is a cloudy layer between the 2 layers after the EZ:faast derivatization, is this normal?
• There is a glass protruding from the injection side of inlet liner AG0-8175; is this normal?
• There is condensation on the EZ:faast SPE tips, is this normal?
• There is small amounts of water in my mobile phase. How do I deal with reproducibility problems on silica columns used in normal phase?
• Today I will see a customer that work only with poroshell. Please do you have some advice to prove that Kinetex is better than poroshell (ID of particules, fabrication ...)
• What Aeris WIDEPORE selectivity should I choose?
• What are chiral isomers? How do I recognize Chiral centers?
• What are hydrolysis yields using 6 N HCl as indicated in the EZ:faast manual?
• What are other factors to consider that can affect my HILIC separation?
• What are secondary interactions in SPE?
• What are shear effects, and what can be done to reduce them?
• What are some general guidelines before beginning method development on my HILIC method?