Ann Montross
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Latest activity by Ann Montross-
Ann Montross created an article,
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Ann Montross created an article,
Lux Amp Series: More tips and tricks
Do not filter the mobile phase to degas the solution. Allow an inline degasser do all the work. Do not run this mobile phase higher than 40⁰
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Ann Montross created an article,
Lux Amp Series: Is the pH of the mobile phase stable?
Yes, if this volatile buffer is in a capped HPLC solvent reservoir, the pH will remain stable The mobile phase is a buffered solution, “a solution that resists changes in pH when acid or alkali is...
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Ann Montross created an article,
Lux Amp Series: Will the high amount of ammonium salts (ammonium bicarbonate and ammonium hydroxide) cause issues with my mass spec detector?
Ammonium Bicarbonate is a volatile buffer which is appropriate for mass spec detectors. A preliminary lifetime study shows no salt build up on the ion source of the mass spec.
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Ann Montross created an article,
Lux Amp Series: How often should I make new mobile phase? Do I need to make completely new mobile phase or can I keep adding new mobile phase to the old mobile phase?
Since Ammonium Bicarbonate is a volatile buffer it is best to make new mobile phase every day but we have found that this buffer is stable for as long as 2 days. It is best practice to make comple...
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Ann Montross created an article,
Lux Amp Series: How much Ammonium Hydroxide do I use to adjust the 5mM Ammonium Bicarbonate solution to pH 11?
Make up a 1 Liter solution of 5 mM Ammonium Bicarbonate and mix well. Transfer to a larger flask or beaker and follow the procedure below. The solution will easily get to pH 9 with a small amount ...