Lux Amp Series: How much Ammonium Hydroxide do I use to adjust the 5mM Ammonium Bicarbonate solution to pH 11?

1. Make up a 1 Liter solution of 5 mM Ammonium Bicarbonate and mix well. Transfer to a larger flask or beaker and follow the procedure below.
2. The solution will easily get to pH 9 with a small amount of concentrated Ammonium Hydroxide. In order to get to pH 11 you should expect to add an amount of concentrated Ammonium Hydroxide around 5-10% of the total volume of your solution.  For example if you were to make a 1 Liter solution of 5 mM Ammonium Bicarbonate then expect to add as much as 50-100 mL of concentrated Ammonium Hydroxide.