Helen Harrington
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Activity overview
Latest activity by Helen Harrington-
Helen Harrington created an article,
What is the shelf life of syringe filters?
The Non-Sterile syringe filters have 5 year shelf life For our Sterile Syringe filters they have a 3 year shelf life
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Helen Harrington created an article,
What should I dissolve my sample in to inject it onto my HPLC column
The optimal sample diluent is to dissolve your sample in your starting mobile phase which ensures the compounds reach the column in their desired state for interaction with the stationary phase. If...
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Helen Harrington created an article,
What is the pressure stability of our 1mm ID columns
For 1mm ID columns packed with 2.6µm, 1.7µm or 1.6µm particles the pressure limit is 15,000 psi For all other 1mm columns packed with 3 and 5 µm materials it is 5000 psi
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Helen Harrington created an article,
What is the recommended flow rate for 1 mm ID, 0.5 and 0.3 mm ID columns?
For 1mm columns it is between 10-100uL/min for 0.3mm columns we would recommend 1-10uL/min and 0.075mm column it is 0.1-1uL/min.
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Helen Harrington created an article,
Why are hybrid silica's preferred for reversed phase analysis of oligonucleotides
Oligonucleotides are inherently polar and therefore not amenable to standard reversed phase HPLC. Purification of oligo’s typically demands reversed phase with aggressive ion pair and modifiers. Wh...
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Helen Harrington created an article,
What is the shipping solvent for bioZen Glycan
bioZen Glycan is shipped in 90% acetonitrile 10% 100mM ammonium formate
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Helen Harrington created an article,
What would be a typical gradient profile for intact analysis of mAbs?
When analyzing intact mAbs using a widepore reversed phase column we would recommend you begin with a short high aqueous hold of around 10-15% 0.1% TFA in water. A typical gradient would then run u...
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Helen Harrington created an article,
Can you use organic solvents in with bioZen SEC or Yarra SEC?
Occasionally to facilitate a true entropic SEC separation a small amount of an organic modified such as ACN is required however more than 15% may result in de-naturing or unfolding of the protein t...
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Helen Harrington created an article,
What is the temperature limit of a column being used in SFC conditions
We would recommend you do not run the column higher than 65C
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Helen Harrington created an article,
How would you recommend storing a column which has been used in SFC mode?
To store the column you need to run a gradient up to 100% modifier (typically methanol) and then store in the modifier