What are typical loadings for BioZen SEC columns?

For size exclusion, there are two considerations - sample volume and sample concentration.
As a general rule no more than 5 % of the column volume should be loaded. Theoretically, a 300 x 4.6 mm column, with a column volume of ~ 5 mL, would limit injection volume to 200 µL. In practice, volumes of 10-30 µL are common.
Another important consideration is sample concentration; the higher the concentration of protein, the higher the viscosity of the sample. This difference in viscosity can lead to peak shape distortion. The distortion can occur either through exclusion effects or a solvent front referred to as “viscous fingering”. A good starting concentration is 1 mg/mL, though optimal concentrations must be determined experimentally