The loading capacity of reversed phase column depends on the property of your analytes as well as the separation you can get. Normally we suggest loading 1% of the sorbent mass. Please see the below chart for more detail.
| 10 mm ID | 21.2 mm ID | 30 mm ID | ||||
| Length (mm) | Column Bed Mass | 1% specific load | Column Bed Mass | 1% specific load | Column Bed Mass | 1% specific load |
| 50 | 2.4g | 24mg | 10.6g | 106mg | 21.2g | 212mg |
| 75 | 3.5g | 35mg | 15.9g | 159mg | 31.8g | 318mg |
| 100 | 4.7g | 47mg | 21.2g | 212mg | 42.4g | 424mg |
| 150 | 7.1g | 71mg | 31.7g | 317mg | 63.6g | 636mg |
| 250 | 11.8g | 118mg | 52.9g | 529mg | 106g | 1060mg |
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