Can Phree be used for phospholipids analysis?

Phree is designed to remove phospholipids and not for phospholipids analysis.  While we cannot guarantee quantitative elution of phospholipids off Phree, you can try applying a 100% high organic elution, i.e. dichloromethane.  This may also dissolve precipitated proteins.  If this is a problem, you can consider doing a protein precipitation first.