The combined effect of all components of the sample, other than the analyte itself, causing a signal enhancement or suppression is recognized as “matrix effect”.
Matrix effect can be removed by sample preparation techniques, which can be as simple as filtering, liquid-liquid extraction or SLE, to more sophisticated ones such as solid phase extraction, SPME, accelerated solvent extraction, microwave-assisted extraction, and supercritical fluid extraction. Matrix effect can also be reduced or eliminated through LC method development by using gradients or alternative column selectivity.
To determine matrix effect, one can do a simple experiment by comparing the extracted analyte response to the non-extracted analyte response.
% Matrix Effect = Extracted Analyte Response X 100%
Non-Extracted Analyte Response
If your customer is unsure whether SPE is necessary, this experiment will help gain more insight to the nature of the sample.
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