How do you pretreat meat samples? – Phenomenex

Sample pretreatment:

Add 1 mL of 1% formic acid aqueous solution to 1 g of meat sample. Shake for 1 min and let stand for 5 minutes. Add 1 mL of methanol to sample mixture. Place on shaker for 15 minutes. Centrifuge. Transfer supernatant to a culture tube and dilute with 3 mL of deionized water. Sample should be acidic at this point. Adjust pH to 6 using NH4OH. This sample mixture will then undergo SPE.