How can I change a tertiary mobile phase gradient to binary?

 

If there are only 2 solvents simply prepare the starting mobile phase conditions as mobile phase ‘A’ and the final mobile phase conditions as ‘B’ and run a gradient from 100% A to 100% B.

For example, taking application 18842, mobile phase A would be 10 mM ammonium formate pH 3.2 in H2O/ACN (10:90) and mobile phase B would be 10 mM ammonium formate pH 3.2 in H2O/ACN (50:50).

 

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