The best way to diagnose whether or not you are having a problem with GC column bleed is by generating a bleed profile when you first install a column, then compare the bleed profile from a more recent run under the same chromatographic conditions.
If there is a substantial increase in column bleed, the column may have been damaged or contaminated. This could be due to a variety of problems including oxygen in the carrier gas and more likely sample residues.
If a GC-MS is available the typical bleed ions for low polarity columns will be m/z 207, 73, 281, 355.
0 Comments