When using Phree, should I add sample first or solvent first?

It is possible to add either sample first or solvent first, however we have found that the best mixing occurs when adding plasma first followed by solvent. It is important to add solvent directly into the sample or vice versa. When solvent or sample is added alongside the wall of the Phree plate, a bilayer will form making it difficult to properly mix the plasma and solvent. Improper mixing will result in incomplete precipitation.

For other questions on Phree products, please refer to our Phree Phospholipid Removal FAQ document.